Composite

Part:BBa_K784034:Design

Designed by: Noa Katz   Group: iGEM12_Technion   (2012-09-18)


pSp6_xyIE reporter gene


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal XhoI site found at 29
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 371
    Illegal NgoMIV site found at 543
    Illegal AgeI site found at 894
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

We had to make sure there aren't any restriction sites in the gene itself before using the XhoI and XmaI restriction enzymes.


Source

The RBS and xyIE genes are from the parts registry, made by iGEM2006_Edinburgh.

The promoter and terminator are from:Temme Karsten, et al. 2012. Modular control of multiple pathways using engineered orthogonal T7 polymerases. Nucleic Acids Research (advance access): 1-9.

References